[HTML][HTML] Differential coupling of the sphingosine 1-phosphate receptors Edg-1, Edg-3, and H218/Edg-5 to the Gi, Gq, and G12 families of heterotrimeric G proteins
Sphingosine 1-phosphate (S1P) is one of several bioactive phospholipids that exert
profound mitogenic and morphogenic actions. Originally characterized as a second
messenger, S1P is now recognized to achieve many of its effects through cell surface, G
protein-coupled receptors. We used a subunit-selective [35 S] GTPγS binding assay to
investigate whether the variety of actions exerted through Edg-1, a recently identified
receptor for S1P, might be achieved through multiple G proteins. We found, employing both …
profound mitogenic and morphogenic actions. Originally characterized as a second
messenger, S1P is now recognized to achieve many of its effects through cell surface, G
protein-coupled receptors. We used a subunit-selective [35 S] GTPγS binding assay to
investigate whether the variety of actions exerted through Edg-1, a recently identified
receptor for S1P, might be achieved through multiple G proteins. We found, employing both …