IL-33 fine tunes mast cell degranulation and chemokine production at the single-cell level
Background Mast cells are versatile key components of allergy and inflammation known to
respond to both innate and adaptive immunologic stimuli. However, the response of
individual mast cells to cumulative stimuli remains poorly understood. Objectives We sought
to dissect mast cell responses at the single-cell level and their potentiation by IL-33.
Methods We monitored mast cell degranulation in real time by exploiting the capacity of
fluorochrome-labeled avidin to stain degranulating cells. During the degranulation process …
respond to both innate and adaptive immunologic stimuli. However, the response of
individual mast cells to cumulative stimuli remains poorly understood. Objectives We sought
to dissect mast cell responses at the single-cell level and their potentiation by IL-33.
Methods We monitored mast cell degranulation in real time by exploiting the capacity of
fluorochrome-labeled avidin to stain degranulating cells. During the degranulation process …