The purpose of this study is to provide an intravital noninvasive multiphoton microscopic
platform for long-term ocular imaging in transgenic fluorescent mice with subcellular
resolution. A multiphoton microscopic system with tunable laser output was employed. We
designed a mouse holder incorporated with stereotaxic motorized stage for in vivo three-
dimensional imaging of ocular surface in 3 transgenic mouse line with fluorescent protein
(FP) expression to visualize distinct structures. With our imaging platform and the expression …