The preparation and characterization of a radioiodinated bacterial lipopolysaccharide
RJ Ulevitch - Immunochemistry, 1978 - Elsevier
RJ Ulevitch
Immunochemistry, 1978•ElsevierRadioiodinated LPS† fromE. coli 0111: B4 has been prepared by reactingp-OH
methylbenzimidate with 0111: B4 LPS at alkaline pH. The resulting LPS derivative has been
radiolabeled with Na 125 I. Specific activities of up to 5 μCi/μg LPS may be obtained by this
technique and significantly the preparation of the radioiodinated LPS does not alter the
biophysical, immunologic or biologic properties of 0111: B4 LPS. The methods described
here are applicable to any 'protein free'LPS preparation containing primary amino groups.
methylbenzimidate with 0111: B4 LPS at alkaline pH. The resulting LPS derivative has been
radiolabeled with Na 125 I. Specific activities of up to 5 μCi/μg LPS may be obtained by this
technique and significantly the preparation of the radioiodinated LPS does not alter the
biophysical, immunologic or biologic properties of 0111: B4 LPS. The methods described
here are applicable to any 'protein free'LPS preparation containing primary amino groups.
Abstract
Radioiodinated LPS† fromE. coli 0111 :B4 has been prepared by reactingp-OH methylbenzimidate with 0111: B4 LPS at alkaline pH. The resulting LPS derivative has been radiolabeled with Na125I. Specific activities of up to 5 μCi/μg LPS may be obtained by this technique and significantly the preparation of the radioiodinated LPS does not alter the biophysical, immunologic or biologic properties of 0111:B4 LPS. The methods described here are applicable to any ‘protein free’ LPS preparation containing primary amino groups.
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