FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells.
L Penter, K Dietze, L Bullinger… - European Journal of …, 2018 - europepmc.org
European Journal of Immunology, 2018•europepmc.org
FACS index sorting allows the isolation of single cells with retrospective identification of
each single cell's high-dimensional immune phenotype. We experimentally determine the
error rate of index sorting and combine the technology with T cell receptor sequencing to
identify clonal T cell expansion in aplastic anemia bone marrow as an example.
each single cell's high-dimensional immune phenotype. We experimentally determine the
error rate of index sorting and combine the technology with T cell receptor sequencing to
identify clonal T cell expansion in aplastic anemia bone marrow as an example.
FACS index sorting allows the isolation of single cells with retrospective identification of each single cell's high-dimensional immune phenotype. We experimentally determine the error rate of index sorting and combine the technology with T cell receptor sequencing to identify clonal T cell expansion in aplastic anemia bone marrow as an example.
europepmc.org