[HTML][HTML] Selective amplification of classical and atypical prions using modified protein misfolding cyclic amplification
N Makarava, R Savtchenko, IV Baskakov - Journal of Biological Chemistry, 2013 - ASBMB
With the development of protein misfolding cyclic amplification (PMCA), the topic of faithful
propagation of prion strain-specific structures has been constantly debated. Here we show
that by subjecting brain material of a synthetic strain consisting of a mixture of self-replicating
states to PMCAb, selective amplification of PrP Sc could be achieved, and that PMCAb
mimicked the evolutionary trend observed during serial transmission in animals. On the
other hand, using modified PMCAb conditions that employ partially deglycosylated PrP C …
propagation of prion strain-specific structures has been constantly debated. Here we show
that by subjecting brain material of a synthetic strain consisting of a mixture of self-replicating
states to PMCAb, selective amplification of PrP Sc could be achieved, and that PMCAb
mimicked the evolutionary trend observed during serial transmission in animals. On the
other hand, using modified PMCAb conditions that employ partially deglycosylated PrP C …