[HTML][HTML] Sialylation controls prion fate in vivo
S Srivastava, E Katorcha, ML Daus, P Lasch… - Journal of Biological …, 2017 - ASBMB
Prions or PrP Sc are proteinaceous infectious agents that consist of misfolded, self-
replicating states of a sialoglycoprotein called the prion protein or PrP C. The current work
tests a new hypothesis that sialylation determines the fate of prions in an organism. To
begin, we produced control PrP Sc from PrP C using protein misfolding cyclic amplification
with beads (PMCAb), and also generated PrP Sc with reduced sialylation levels using the
same method but with partially desialylated PrP C as a substrate (dsPMCAb). Syrian …
replicating states of a sialoglycoprotein called the prion protein or PrP C. The current work
tests a new hypothesis that sialylation determines the fate of prions in an organism. To
begin, we produced control PrP Sc from PrP C using protein misfolding cyclic amplification
with beads (PMCAb), and also generated PrP Sc with reduced sialylation levels using the
same method but with partially desialylated PrP C as a substrate (dsPMCAb). Syrian …