[HTML][HTML] Multiplex SILAC analysis of a cellular TDP-43 proteinopathy model reveals protein inclusions associated with SUMOylation and diverse polyubiquitin chains
Transactive response (TAR) DNA-binding protein 43 (TDP-43) is a major protein component
within ubiquitin-positive inclusions of frontotemporal lobar degeneration and amyotrophic
lateral sclerosis. Although TDP-43 is a nuclear DNA/RNA-binding protein, in pathological
conditions, TDP-43 has been reported to redistribute to the cytoplasm where it is cleaved
and forms insoluble, ubiquitinated, and phosphorylated inclusions. Here we present a
cellular model in which full-length human TDP-43 or a splicing isoform (TDP-S6) that lacks …
within ubiquitin-positive inclusions of frontotemporal lobar degeneration and amyotrophic
lateral sclerosis. Although TDP-43 is a nuclear DNA/RNA-binding protein, in pathological
conditions, TDP-43 has been reported to redistribute to the cytoplasm where it is cleaved
and forms insoluble, ubiquitinated, and phosphorylated inclusions. Here we present a
cellular model in which full-length human TDP-43 or a splicing isoform (TDP-S6) that lacks …