[HTML][HTML] Identification of CRM1-dependent nuclear export cargos using quantitative mass spectrometry
Chromosome region maintenance 1/exportin1/Exp1/Xpo1 (CRM1) is the major transport
receptor for the export of proteins from the nucleus. It binds to nuclear export signals (NESs)
that are rich in leucines and other hydrophobic amino acids. The prediction of NESs is
difficult because of the extreme recognition flexibility of CRM1. Furthermore, proteins can be
exported upon binding to an NES-containing adaptor protein. Here we present an approach
for identifying targets of the CRM1-export pathway via quantitative mass spectrometry using …
receptor for the export of proteins from the nucleus. It binds to nuclear export signals (NESs)
that are rich in leucines and other hydrophobic amino acids. The prediction of NESs is
difficult because of the extreme recognition flexibility of CRM1. Furthermore, proteins can be
exported upon binding to an NES-containing adaptor protein. Here we present an approach
for identifying targets of the CRM1-export pathway via quantitative mass spectrometry using …