[HTML][HTML] Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge
N Pardi, AJ Secreto, X Shan, F Debonera… - Nature …, 2017 - nature.com
N Pardi, AJ Secreto, X Shan, F Debonera, J Glover, Y Yi, H Muramatsu, H Ni, BL Mui…
Nature communications, 2017•nature.comMonoclonal antibodies are one of the fastest growing classes of pharmaceutical products,
however, their potential is limited by the high cost of development and manufacturing. Here
we present a safe and cost-effective platform for in vivo expression of therapeutic antibodies
using nucleoside-modified mRNA. To demonstrate feasibility and protective efficacy,
nucleoside-modified mRNAs encoding the light and heavy chains of the broadly neutralizing
anti-HIV-1 antibody VRC01 are generated and encapsulated into lipid nanoparticles …
however, their potential is limited by the high cost of development and manufacturing. Here
we present a safe and cost-effective platform for in vivo expression of therapeutic antibodies
using nucleoside-modified mRNA. To demonstrate feasibility and protective efficacy,
nucleoside-modified mRNAs encoding the light and heavy chains of the broadly neutralizing
anti-HIV-1 antibody VRC01 are generated and encapsulated into lipid nanoparticles …
Abstract
Monoclonal antibodies are one of the fastest growing classes of pharmaceutical products, however, their potential is limited by the high cost of development and manufacturing. Here we present a safe and cost-effective platform for in vivo expression of therapeutic antibodies using nucleoside-modified mRNA. To demonstrate feasibility and protective efficacy, nucleoside-modified mRNAs encoding the light and heavy chains of the broadly neutralizing anti-HIV-1 antibody VRC01 are generated and encapsulated into lipid nanoparticles. Systemic administration of 1.4 mg kg−1 of mRNA into mice results in ∼170 μg ml−1 VRC01 antibody concentrations in the plasma 24 h post injection. Weekly injections of 1 mg kg−1 of mRNA into immunodeficient mice maintain trough VRC01 levels above 40 μg ml−1. Most importantly, the translated antibody from a single injection of VRC01 mRNA protects humanized mice from intravenous HIV-1 challenge, demonstrating that nucleoside-modified mRNA represents a viable delivery platform for passive immunotherapy against HIV-1 with expansion to a variety of diseases.
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