[HTML][HTML] The Expression Level of mRNA, Protein, and DNA Methylation Status of FOSL2 of Uyghur in XinJiang in Type 2 Diabetes
J Li, S Li, Y Hu, G Cao, S Wang, P Rai… - Journal of Diabetes …, 2016 - hindawi.com
J Li, S Li, Y Hu, G Cao, S Wang, P Rai, X Wang, K Sun
Journal of Diabetes Research, 2016•hindawi.comObjective. We investigated the expression levels of both FOSL2 mRNA and protein as well
as evaluating DNA methylation in the blood of type 2 diabetes mellitus (T2DM) Uyghur
patients from Xinjiang. This study also evaluated whether FOSL2 gene expression had
demonstrated any associations with clinical and biochemical indicators of T2DM. Methods.
One hundred Uyghur subjects where divided into two groups, T2DM and nonimpaired
glucose tolerance (NGT) groups. DNA methylation of FOSL2 was also analyzed by …
as evaluating DNA methylation in the blood of type 2 diabetes mellitus (T2DM) Uyghur
patients from Xinjiang. This study also evaluated whether FOSL2 gene expression had
demonstrated any associations with clinical and biochemical indicators of T2DM. Methods.
One hundred Uyghur subjects where divided into two groups, T2DM and nonimpaired
glucose tolerance (NGT) groups. DNA methylation of FOSL2 was also analyzed by …
Objective
We investigated the expression levels of both FOSL2 mRNA and protein as well as evaluating DNA methylation in the blood of type 2 diabetes mellitus (T2DM) Uyghur patients from Xinjiang. This study also evaluated whether FOSL2 gene expression had demonstrated any associations with clinical and biochemical indicators of T2DM.
Methods
One hundred Uyghur subjects where divided into two groups, T2DM and nonimpaired glucose tolerance (NGT) groups. DNA methylation of FOSL2 was also analyzed by MassARRAY Spectrometry and methylation data of individual units were generated by the EpiTyper v1.0.5 software. The expression levels of FOS-like antigen 2 (FOSL2) and the protein expression levels were analyzed.
Results
Significant differences were observed in mRNA and protein levels when compared with the NGT group, while methylation rates of eight CpG units within the FOSL2 gene were higher in the T2DM group. Methylation of CpG sites was found to inversely correlate with expression of other markers.
Conclusions
Results show that a correlation between mRNA, protein, and DNA methylation of FOSL2 gene exists among T2DM patients from Uyghur. FOSL2 protein and mRNA were downregulated and the DNA became hypermethylated, all of which may be involved in T2DM pathogenesis in this population.
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