[HTML][HTML] Cell cycle dependence of DNA-dependent protein kinase phosphorylation in response to DNA double strand breaks
DNA-dependent protein kinase (DNA-PK), consisting of Ku and DNA-PKcs subunits, is the
key component of the non-homologous end-joining (NHEJ) pathway of DNA double strand
break (DSB) repair. Although the kinase activity of DNA-PKcs is essential for NHEJ, thus far,
no in vivo substrate has been conclusively identified except for an autophosphorylation site
on DNA-PKcs itself (threonine 2609). Here we report the ionizing radiation (IR)-induced
autophosphorylation of DNA-PKcs at a novel site, serine 2056, the phosphorylation of which …
key component of the non-homologous end-joining (NHEJ) pathway of DNA double strand
break (DSB) repair. Although the kinase activity of DNA-PKcs is essential for NHEJ, thus far,
no in vivo substrate has been conclusively identified except for an autophosphorylation site
on DNA-PKcs itself (threonine 2609). Here we report the ionizing radiation (IR)-induced
autophosphorylation of DNA-PKcs at a novel site, serine 2056, the phosphorylation of which …