[HTML][HTML] Efficacy of ImageJ in the assessment of apoptosis
IM Helmy, AM Abdel Azim - Diagnostic pathology, 2012 - Springer
IM Helmy, AM Abdel Azim
Diagnostic pathology, 2012•SpringerObjective To verify the efficacy of ImageJ 1.43 n in determining the extent of apoptosis which
is a complex and multistep process. Study Design Cisplatin in different concentrations was
used to induce apoptosis in cultured Hep2 cells. Cell viability assay and nuclear image
analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected
to be undergoing apoptosis from control cells based on parameters such as nuclear area,
circularity, perimeter and nuclear area factor (NAF), in association with visual morphology …
is a complex and multistep process. Study Design Cisplatin in different concentrations was
used to induce apoptosis in cultured Hep2 cells. Cell viability assay and nuclear image
analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected
to be undergoing apoptosis from control cells based on parameters such as nuclear area,
circularity, perimeter and nuclear area factor (NAF), in association with visual morphology …
Objective
To verify the efficacy of ImageJ 1.43 n in determining the extent of apoptosis which is a complex and multistep process.
Study Design
Cisplatin in different concentrations was used to induce apoptosis in cultured Hep2 cells. Cell viability assay and nuclear image analysis of stained Hep2 cells were used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells based on parameters such as nuclear area, circularity, perimeter and nuclear area factor (NAF), in association with visual morphology.
Results
Image analysis revealed a progressive and highly significant decrease in nuclear area factor detected in apoptotic cells and in cells suspected of undergoing apoptosis compared to the control cells (P-values < 0.01). Some of the other studied parameters showed also the same trend. This decrease was assumed to indicate DNA loss. Image analysis results correlated positively and significantly with the results obtained by cell viability assay (R = 0.958, P-value = 0.042). NAF was the most reliable parameter in assessment of apoptosis.
Conclusion
Nuclear area factor can be calculated using powerful free and open-source software. Consequently, a quantitative measure of apoptosis can be obtained that is linked to morphological changes. ImageJ 1.43 n may therefore provide a useful tool for the assessment and discrimination of apoptotic cells.
Virtual slides
The virtual slide(s) for this article can be found here:
http://www.diagnosticpathology.diagnomx.eu/vs/5929043086367338
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