Optimization of the wound scratch assay to detect changes in murine mesenchymal stromal cell migration after damage by soluble cigarette smoke extract

N Cormier, A Yeo, E Fiorentino, J Paxson - JoVE (Journal of Visualized …, 2015 - jove.com
Cell migration is vital to many physiological and pathological processes including tissue
development, repair, and regeneration, cancer metastasis, and inflammatory responses.
Given the current interest in the role of mesenchymal stromal cells in mediating tissue repair,
we are interested in quantifying the migratory capacity of these cells, and understanding
how migratory capacity may be altered after damage. Optimization of a rigorously
quantitative migration assay that is both easy to customize and cost-effective to perform is …