Immune Distribution and Localization of Phosphoantigen-Specific Vγ2Vδ2 T Cells in Lymphoid and Nonlymphoid Tissues in Mycobacterium tuberculosis Infection
D Huang, Y Shen, L Qiu, CY Chen, L Shen… - Infection and …, 2008 - Am Soc Microbiol
D Huang, Y Shen, L Qiu, CY Chen, L Shen, J Estep, R Hunt, D Vasconcelos, G Du, P Aye…
Infection and immunity, 2008•Am Soc MicrobiolLittle is known about the immune distribution and localization of antigen-specific T cells in
mucosal interfaces of tissues/organs during infection of humans. In this study, we made use
of a macaque model of Mycobacterium tuberculosis infection to assess phosphoantigen-
specific Vγ2Vδ2 T cells regarding their tissue distribution, anatomical localization, and
correlation with the presence or absence of tuberculosis (TB) lesions in lymphoid and
nonlymphoid organs/tissues in the progression of severe pulmonary TB. Progression of …
mucosal interfaces of tissues/organs during infection of humans. In this study, we made use
of a macaque model of Mycobacterium tuberculosis infection to assess phosphoantigen-
specific Vγ2Vδ2 T cells regarding their tissue distribution, anatomical localization, and
correlation with the presence or absence of tuberculosis (TB) lesions in lymphoid and
nonlymphoid organs/tissues in the progression of severe pulmonary TB. Progression of …
Abstract
Little is known about the immune distribution and localization of antigen-specific T cells in mucosal interfaces of tissues/organs during infection of humans. In this study, we made use of a macaque model of Mycobacterium tuberculosis infection to assess phosphoantigen-specific Vγ2Vδ2 T cells regarding their tissue distribution, anatomical localization, and correlation with the presence or absence of tuberculosis (TB) lesions in lymphoid and nonlymphoid organs/tissues in the progression of severe pulmonary TB. Progression of pulmonary M. tuberculosis infection generated diverse distribution patterns of Vγ2Vδ2 T cells, with remarkable accumulation of these cells in lungs, bronchial lymph nodes, spleens, and remote nonlymphoid organs but not in blood. Increased numbers of Vγ2Vδ2 T cells in tissues were associated with M. tuberculosis infection but were independent of the severity of TB lesions. In lungs with apparent TB lesions, Vγ2Vδ2 T cells were present within TB granulomas. In extrathoracic organs, Vγ2Vδ2 T cells were localized in the interstitial compartment of nonlymphoid tissues, and the interstitial localization was present despite the absence of detectable TB lesions. Finally, Vγ2Vδ2 T cells accumulated in tissues appeared to possess cytokine production function, since granzyme B was detectable in the γδ T cells present within granulomas. Thus, clonally expanded Vγ2Vδ2 T cells appeared to undergo trans-endothelial migration, interstitial localization, and granuloma infiltration as immune responses to M. tuberculosis infection.
American Society for Microbiology