[HTML][HTML] Phosphorylation and regulation of DNA ligase IV stability by DNA-dependent protein kinase
YG Wang, C Nnakwe, WS Lane, M Modesti… - Journal of Biological …, 2004 - ASBMB
DNA ligase IV (Lig4), x-ray cross-complementation group 4 (XRCC4), and DNA-dependent
protein kinase (DNA-PK) are essential mammalian nonhomologous end joining proteins
used for V (D) J recombination and DNA repair. Previously a Lig4 peptide was reported to
be an in vitro substrate for DNA-PK, but the phosphorylation state of Lig4 protein in vivo is
not known. In this study, we report that a full-length Lig4 construct was expressed as a
phosphoprotein in the cell. Also the full-length Lig4 protein, in complex with XRCC4, was an …
protein kinase (DNA-PK) are essential mammalian nonhomologous end joining proteins
used for V (D) J recombination and DNA repair. Previously a Lig4 peptide was reported to
be an in vitro substrate for DNA-PK, but the phosphorylation state of Lig4 protein in vivo is
not known. In this study, we report that a full-length Lig4 construct was expressed as a
phosphoprotein in the cell. Also the full-length Lig4 protein, in complex with XRCC4, was an …