Quantification of apoptosis and necroptosis at the single cell level by a combination of Imaging Flow Cytometry with classical Annexin V/propidium iodide staining
S Pietkiewicz, JH Schmidt, IN Lavrik - Journal of immunological methods, 2015 - Elsevier
Precisely identifying the type of programmed cell death is one of the key questions in
contemporary biomedical research. We developed a straightforward approach allowing
quantitative discrimination between two types of cell death on the single cell level: apoptosis
and necroptosis. This method uses the combination of imaging flow cytometry with classical
Annexin V/propidium iodide staining, which allows for the ascertainment of typical features
of dying cells: exposure of the phospholipid phosphatidylserine and the loss of membrane …
contemporary biomedical research. We developed a straightforward approach allowing
quantitative discrimination between two types of cell death on the single cell level: apoptosis
and necroptosis. This method uses the combination of imaging flow cytometry with classical
Annexin V/propidium iodide staining, which allows for the ascertainment of typical features
of dying cells: exposure of the phospholipid phosphatidylserine and the loss of membrane …