Endothelial cell adhesion to the extracellular matrix induces c-Src–dependent VEGFR-3 phosphorylation without the activation of the receptor intrinsic kinase activity

F Galvagni, S Pennacchini, A Salameh… - Circulation …, 2010 - Am Heart Assoc
F Galvagni, S Pennacchini, A Salameh, M Rocchigiani, F Neri, M Orlandini, F Petraglia
Circulation research, 2010Am Heart Assoc
Rationale: Integrins cooperate with growth factor receptors to promote downstream signaling
for cell proliferation and migration. However, the mechanism of receptor activation is still
unknown. Objective: To analyze the mechanism of phosphorylation of the vascular
endothelial growth factor receptor (VEGFR)-3 by cell adhesion. Methods and Results: We
show that VEGFR-3 phosphorylation, induced by cell attachment to the extracellular matrix,
is independent from the intrinsic kinase activity of the receptor, as evidenced from …
Rationale: Integrins cooperate with growth factor receptors to promote downstream signaling for cell proliferation and migration. However, the mechanism of receptor activation is still unknown.
Objective: To analyze the mechanism of phosphorylation of the vascular endothelial growth factor receptor (VEGFR)-3 by cell adhesion.
Methods and Results: We show that VEGFR-3 phosphorylation, induced by cell attachment to the extracellular matrix, is independent from the intrinsic kinase activity of the receptor, as evidenced from phosphorylation cell adhesion experiments with a mutant kinase dead receptor or in the presence of the specific kinase inhibitor MAZ 51. Cell adhesion experiments in the presence of the c-Src inhibitor PP2 or in fibroblast triple knockout for c-Src, Yes, and Fyn (SYF) demonstrate that VEGFR-3 phosphorylation, induced by extracellular matrix, is mediated by c-Src. Kinase assays in vitro with recombinant c-Src show that VEGFR-3 is a direct c-Src target and mass spectrometry analysis identified the sites phosphorylated by c-Src as tyrosine 830, 833, 853, 1063, 1333, and 1337, demonstrating that integrin-mediated receptor phosphorylation induces a phosphorylation pattern that is distinct from that induced by growth factors. Furthermore, pull-down assays show that integrin-mediated VEGFR-3 phosphorylation activates the recruitment to the receptor of the adaptor proteins CRKI/II and SHC inducing activation of JNK.
Conclusions: These data suggest that cell adhesion to extracellular matrix induces a downstream signaling using the tyrosine kinase receptor VEGFR-3 as scaffold.
Am Heart Assoc