Human mast cell carboxypeptidase. Selective localization to MCTC cells.

AM Irani, SM Goldstein, BU Wintroub… - … (Baltimore, Md.: 1950 …, 1991 - journals.aai.org
AM Irani, SM Goldstein, BU Wintroub, T Bradford, LB Schwartz
Journal of immunology (Baltimore, Md.: 1950), 1991journals.aai.org
Two murine mAb were prepared against human mast cell carboxypeptidase (HMC-CP)
purified from human skin, and were termed CP1 and CP2, respectively. Double
immunohistochemical labeling of Carnoy's-fixed sections of human skin, lung, and
gastrointestinal tissue with CP1 and CP2, respectively, and with a murine monoclonal
antitryptase antibody demonstrated that HMC-CP was selectively present in a subset of
human mast cells. Double labeling experiments with CP1 and CP2, respectively, and a …
Abstract
Two murine mAb were prepared against human mast cell carboxypeptidase (HMC-CP) purified from human skin, and were termed CP1 and CP2, respectively. Double immunohistochemical labeling of Carnoy's-fixed sections of human skin, lung, and gastrointestinal tissue with CP1 and CP2, respectively, and with a murine monoclonal antitryptase antibody demonstrated that HMC-CP was selectively present in a subset of human mast cells. Double labeling experiments with CP1 and CP2, respectively, and a murine anti-chymase mAb demonstrated the presence of HMC-CP in the tryptase-positive, chymase-positive mast cell type (MCTC) only. Immunohistochemical labeling of peripheral blood leukocytes resulted in staining of monocytes with CP2 but not with CP1. In addition to chymase and a cathepsin-G like proteinase, HMC-CP is another neutral protease that is selectively present in the MCTC tryptase-positive, chymase-positive mast cells type of mast cell, thus extending the biochemical definition of human mast cell heterogeneity.
journals.aai.org