Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI
TM Laidlaw, JW Steinke, AM Tiñana, C Feng… - Journal of allergy and …, 2011 - Elsevier
BACKGROUND: Studies of human mast cells (MCs) are constrained by the paucity of
functional cell lines, the expense of maintaining MCs in culture, and technical complexities.
OBJECTIVE: We derived and characterized a human MC line that arose spontaneously from
a culture of nontransformed hematopoietic progenitor cells. METHODS: CD34+ enriched
mononuclear cells derived from a donor with aspirin-exacerbated respiratory disease were
cultured for 8 weeks with stem cell factor and IL-6 and with IL-3 for the first week only. The …
functional cell lines, the expense of maintaining MCs in culture, and technical complexities.
OBJECTIVE: We derived and characterized a human MC line that arose spontaneously from
a culture of nontransformed hematopoietic progenitor cells. METHODS: CD34+ enriched
mononuclear cells derived from a donor with aspirin-exacerbated respiratory disease were
cultured for 8 weeks with stem cell factor and IL-6 and with IL-3 for the first week only. The …