Ovca1 regulates cell proliferation, embryonic development, and tumorigenesis

CM Chen, RR Behringer - Genes & development, 2004 - genesdev.cshlp.org
Genes & development, 2004genesdev.cshlp.org
Loss of OVCA1/DPH2L1 correlates with ovarian and breast cancer. To study its in vivo role,
we generated Ovca1 mutant alleles in mice. Ovca1 heterozygotes spontaneously develop
cancer. Ovca1 mutant mice die during embryonic development and at birth with
developmental delay and defects in multiple organ systems. Cell proliferation defects were
observed in Ovca1 mutant mouse embryonic fibroblasts (MEFs). p53 deficiency can rescue
these Ovca1 mutant MEF proliferation defects and partially rescue Ovca1 mutant embryonic …
Loss of OVCA1/DPH2L1 correlates with ovarian and breast cancer. To study its in vivo role, we generated Ovca1 mutant alleles in mice. Ovca1 heterozygotes spontaneously develop cancer. Ovca1 mutant mice die during embryonic development and at birth with developmental delay and defects in multiple organ systems. Cell proliferation defects were observed in Ovca1 mutant mouse embryonic fibroblasts (MEFs). p53 deficiency can rescue these Ovca1 mutant MEF proliferation defects and partially rescue Ovca1 mutant embryonic phenotypes. Furthermore, Ovca1; p53 double heterozygotes developed tumors quicker than p53 heterozygotes and with an increased carcinoma incidence. Multiple tumor burden in Ovca1 heterozygotes that were also p53 deficient was significantly higher than in p53 homozygous mutants. These in vivo findings demonstrate that Ovca1 is a tumor suppressor that can modify p53-induced tumorigenesis and suggest that it acts as a positive regulator for cell cycle progression. The close linkage of OVCA1 and p53 on human Chromosome 17 suggests that coordinated loss may be an important mechanism for the evolution of ovarian, breast, and other tumor phenotypes.
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