Suprachoroidal drug delivery to the back of the eye using hollow microneedles
SR Patel, ASP Lin, HF Edelhauser… - Pharmaceutical …, 2011 - Springer
SR Patel, ASP Lin, HF Edelhauser, MR Prausnitz
Pharmaceutical research, 2011•SpringerPurpose In this work, we tested the hypothesis that microneedles provide a minimally
invasive method to inject particles into the suprachoroidal space for drug delivery to the back
of the eye. Methods A single, hollow microneedle was inserted into the sclera, and infused
nanoparticle and microparticle suspensions into the suprachoroidal space. Experiments
were performed on whole rabbit, pig, and human eyes ex vivo. Particle delivery was imaged
using brightfield and fluorescence microscopy as well as microcomputed tomography …
invasive method to inject particles into the suprachoroidal space for drug delivery to the back
of the eye. Methods A single, hollow microneedle was inserted into the sclera, and infused
nanoparticle and microparticle suspensions into the suprachoroidal space. Experiments
were performed on whole rabbit, pig, and human eyes ex vivo. Particle delivery was imaged
using brightfield and fluorescence microscopy as well as microcomputed tomography …
Purpose
In this work, we tested the hypothesis that microneedles provide a minimally invasive method to inject particles into the suprachoroidal space for drug delivery to the back of the eye.
Methods
A single, hollow microneedle was inserted into the sclera, and infused nanoparticle and microparticle suspensions into the suprachoroidal space. Experiments were performed on whole rabbit, pig, and human eyes ex vivo. Particle delivery was imaged using brightfield and fluorescence microscopy as well as microcomputed tomography.
Results
Microneedles were shown to deliver sulforhodamine B as well as nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig, and human eyes. Volumes up to 35 μL were administered consistently. Optimization of the delivery device parameters showed that microneedle length, pressure, and particle size played an important role in determining successful delivery into the suprachoroidal space. Needle lengths of 800–1,000 μm and applied pressures of 250–300 kPa provided most reliable delivery.
Conclusions
Microneedles were shown for the first time to deliver nanoparticle and microparticle suspensions into the suprachoroidal space of rabbit, pig and human eyes. This shows that microneedles may provide a minimally invasive method for controlled drug delivery to the back of the eye.
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