[HTML][HTML] Interleukin-6 induces impairment in human subcutaneous adipogenesis in obesity-associated insulin resistance

S Almuraikhy, W Kafienah, M Bashah, I Diboun… - Diabetologia, 2016 - Springer
Diabetologia, 2016Springer
Aims/hypothesis A subset of obese individuals remains insulin sensitive by mechanisms as
yet unclear. The hypothesis that maintenance of normal subcutaneous (SC) adipogenesis
accounts, at least partially, for this protective phenotype and whether it can be abrogated by
chronic exposure to IL-6 was investigated. Methods Adipose tissue biopsies were collected
from insulin-sensitive (IS) and insulin-resistant (IR) individuals undergoing weight-reduction
surgery. Adipocyte size, pre-adipocyte proportion of stromal vascular fraction (SVF)-derived …
Aims/hypothesis
A subset of obese individuals remains insulin sensitive by mechanisms as yet unclear. The hypothesis that maintenance of normal subcutaneous (SC) adipogenesis accounts, at least partially, for this protective phenotype and whether it can be abrogated by chronic exposure to IL-6 was investigated.
Methods
Adipose tissue biopsies were collected from insulin-sensitive (IS) and insulin-resistant (IR) individuals undergoing weight-reduction surgery. Adipocyte size, pre-adipocyte proportion of stromal vascular fraction (SVF)-derived cells, adipogenic capacity and gene expression profiles of isolated pre-adipocytes were determined, along with local in vitro IL-6 secretion. Adipogenic capacity was further assessed in response to exogenous IL-6 application.
Results
Despite being equally obese, IR individuals had significantly lower plasma leptin and adiponectin levels and higher IL-6 levels compared with age-matched IS counterparts. Elevated systemic IL-6 in IR individuals was associated with hyperplasia of adipose tissue-derived SVF cells, despite higher frequency of hypertrophied adipocytes. SC pre-adipocytes from these tissues exhibited lower adipogenic capacity accompanied by downregulation of PPARγ (also known as PPARG) and CEBPα (also known as CEBPA) and upregulation of GATA3 expression. Impaired adipogenesis in IR individuals was further associated with increased adipose secretion of IL-6. Treatment of IS-derived SC pre-adipocytes with IL-6 reduced their adipogenic capacity to levels of the IR group.
Conclusions/interpretation
Obesity-associated insulin resistance is marked by impaired SC adipogenesis, mediated, at least in a subset of individuals, by elevated local levels of IL-6. Understanding the molecular mechanisms underlying reduced adipogenic capacity in IR individuals could help target appropriate therapeutic strategies aimed at those at greatest risk of insulin resistance and type 2 diabetes mellitus.
Springer