Integration and stable germ line transmission of genes injected into mouse pronuclei

JW Gordon, FH Ruddle - Science, 1981 - science.org
JW Gordon, FH Ruddle
Science, 1981science.org
Genetic material has been successfully transferred into the genomes of newborn mice by
injection of that material into pronuclei of fertilized eggs. Initial results indicated two patterns
of processing the injected DNA: one in which the material was not integrated into the host
genome, and another in which the injected genes became associated with high molecular
weight DNA. These patterns are maintained through further development to adulthood. The
evidence presented indicates the covalent association of injected DNA with host sequences …
Genetic material has been successfully transferred into the genomes of newborn mice by injection of that material into pronuclei of fertilized eggs. Initial results indicated two patterns of processing the injected DNA: one in which the material was not integrated into the host genome, and another in which the injected genes became associated with high molecular weight DNA. These patterns are maintained through further development to adulthood. The evidence presented indicates the covalent association of injected DNA with host sequences, and transmission of such linked sequences in a Mendelian distribution to two succeeding generations of progeny.
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