[HTML][HTML] Disruption of the Raf-1-Hsp90 Molecular Complex Results in Destabilization of Raf-1 and Loss of Raf-1-Ras Association (∗)
TW Schulte, MV Blagosklonny, C Ingui… - Journal of Biological …, 1995 - ASBMB
Cytosolic Raf-1 exists in a high molecular weight complex with the heat shock protein
Hsp90, the purpose of which is unknown. The benzoquinone ansamycin, geldanamycin,
specifically binds to Hsp90 and disrupts certain multimolecular complexes containing this
protein. Using this drug, we are able to demonstrate rapid dissociation of both Raf-1-Hsp90
and Raf-1-Ras multimolecular complexes, concomitant with a markedly decreased half-life
of the Raf-1 protein. Continued disruption of the Raf-1-Hsp90 complex results in apparent …
Hsp90, the purpose of which is unknown. The benzoquinone ansamycin, geldanamycin,
specifically binds to Hsp90 and disrupts certain multimolecular complexes containing this
protein. Using this drug, we are able to demonstrate rapid dissociation of both Raf-1-Hsp90
and Raf-1-Ras multimolecular complexes, concomitant with a markedly decreased half-life
of the Raf-1 protein. Continued disruption of the Raf-1-Hsp90 complex results in apparent …