Cytochemical detection of peroxisomes and mitochondria

NA Bonekamp, M Islinger, MG Lázaro… - Cell Imaging Techniques …, 2013 - Springer
NA Bonekamp, M Islinger, MG Lázaro, M Schrader
Cell Imaging Techniques: Methods and Protocols, 2013Springer
Peroxisomes and mitochondria are essential subcellular organelles in mammals.
Interestingly, recent studies have elucidated that these highly dynamic and plastic
organelles exhibit a much closer interrelationship than previously assumed. Peroxisomes
and mitochondria are metabolically linked organelles, which are cooperating and cross-
talking. They share key components of their division machinery and cooperate in antiviral
signaling and defense. As peroxisomal alterations in metabolism, biogenesis, dynamics …
Abstract
Peroxisomes and mitochondria are essential subcellular organelles in mammals. Interestingly, recent studies have elucidated that these highly dynamic and plastic organelles exhibit a much closer interrelationship than previously assumed. Peroxisomes and mitochondria are metabolically linked organelles, which are cooperating and cross-talking. They share key components of their division machinery and cooperate in antiviral signaling and defense. As peroxisomal alterations in metabolism, biogenesis, dynamics, and proliferation have the potential to influence mitochondrial morphology and functions (and vice versa), there is currently great interest in the detection of both organelles under different experimental conditions. Here, we present protocols used successfully in our laboratory for the dual detection of peroxisomes and mitochondria in cultured mammalian cells. We address double immunofluorescence and fluorescence-based techniques as well as reagents to investigate organelle dynamics, morphological alterations, and organelle-specific targeting of proteins. In addition, we describe the application of diaminobenzidine cytochemistry on cultured cells to specifically label peroxisomes in ultrastructural studies.
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