[HTML][HTML] Processing of blood samples influences PBMC viability and outcome of cell-mediated immune responses in antiretroviral therapy-naive HIV-1-infected …

P Bourguignon, F Clément, F Renaud, V Le Bras… - Journal of …, 2014 - Elsevier
Intracellular cytokine staining (ICS) assay is increasingly used in vaccine clinical trials to
measure antigen-specific T-cell mediated immune (CMI) responses in cryopreserved
peripheral blood mononuclear cells (PBMCs) and whole blood. However, recent
observations indicate that several parameters involved in blood processing can impact
PBMC viability and CMI responses, especially in antiretroviral therapy (ART)-naïve HIV-1-
infected individuals. In this phase I study (NCT01610427), we collected blood samples from …