Endocytosis and degradative sorting of NMDA receptors by conserved membrane-proximal signals

DB Scott, I Michailidis, Y Mu, D Logothetis… - Journal of …, 2004 - Soc Neuroscience
DB Scott, I Michailidis, Y Mu, D Logothetis, MD Ehlers
Journal of Neuroscience, 2004Soc Neuroscience
Regulation of the abundance of NMDA receptors (NMDARs) at excitatory synapses is critical
during changes in synaptic efficacy underlying learning and memory as well as during
synapse formation throughout neural development. However, the molecular signals that
govern NMDAR delivery, maintenance, and internalization remain unclear. In this study, we
identify a conserved family of membrane-proximal endocytic signals, two within the NMDAR
type 1 (NR1) subunit and one within the NR2A and NR2B subunits, necessary and sufficient …
Regulation of the abundance of NMDA receptors (NMDARs) at excitatory synapses is critical during changes in synaptic efficacy underlying learning and memory as well as during synapse formation throughout neural development. However, the molecular signals that govern NMDAR delivery, maintenance, and internalization remain unclear. In this study, we identify a conserved family of membrane-proximal endocytic signals, two within the NMDAR type 1 (NR1) subunit and one within the NR2A and NR2B subunits, necessary and sufficient to drive the internalization of NMDARs. These endocytic motifs reside in the region of NMDAR subunits immediately after the fourth membrane segment, a region implicated in use-dependent rundown and NMDA channel inactivation. Although endocytosis driven by the distal C-terminal domain of NR2B is followed by rapid recycling, internalization mediated by membrane-proximal motifs selectively targets receptors to late endosomes and accelerates degradation. These results define a novel conserved signature of NMDARs regulating internalization and postendocytic trafficking.
Soc Neuroscience