Intracellular IL-10 detection in T cells by flowcytometry: the use of protein transport inhibitors revisited
AH Muris, J Damoiseaux, J Smolders… - Journal of …, 2012 - Elsevier
In the past two decades, interleukin-10 (IL-10) has gained much attention as an important
regulatory cytokine involved in self-tolerance. Functional assessment of IL-10 producing
immune cells is traditionally done by stimulation and measurement of cytokine production by
flowcytometry. Thereby a protein transport inhibitor like monensin is used to accumulate the
cytokine of interest intracellularly. In this study we elaborated on the monensin effect on
cytokine detection and focused on IL-10 detection in human T cells. Peripheral blood …
regulatory cytokine involved in self-tolerance. Functional assessment of IL-10 producing
immune cells is traditionally done by stimulation and measurement of cytokine production by
flowcytometry. Thereby a protein transport inhibitor like monensin is used to accumulate the
cytokine of interest intracellularly. In this study we elaborated on the monensin effect on
cytokine detection and focused on IL-10 detection in human T cells. Peripheral blood …