Development and evaluation of a novel, semiautomated Clostridium difficile typing platform
LF Westblade, RR Chamberland… - Journal of clinical …, 2013 - Am Soc Microbiol
LF Westblade, RR Chamberland, D MacCannell, R Collins, ER Dubberke, WM Dunne Jr…
Journal of clinical microbiology, 2013•Am Soc MicrobiolWe describe a novel, semiautomated Clostridium difficile typing platform that is based on
PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform
is reproducible with minimal intra-or interassay variability. This method exhibited a
discriminatory index of 0.954 and is therefore comparable to more arduous typing systems,
such as pulsed-field gel electrophoresis.
PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform
is reproducible with minimal intra-or interassay variability. This method exhibited a
discriminatory index of 0.954 and is therefore comparable to more arduous typing systems,
such as pulsed-field gel electrophoresis.
Abstract
We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsed-field gel electrophoresis.
American Society for Microbiology