Fibroblastic reticular cells (FRCs) are essential players during adaptive immune responses
not only as a structural support for the encounter of antigen-presenting cells and naive T
lymphocytes but also as a source of modulatory signals. However, little is known about this
cell population in humans. To address the phenotypical and functional analysis of human
FRCs here we established splenic (SP) and mesenteric lymph node (LN) CD45-CD31-
CD90+ podoplanin+ myofibroblastic cell cultures. They shared the phenotypical …