[HTML][HTML] Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum
P Mukherjee, G Hough, A Chattopadhyay… - Journal of lipid …, 2017 - ASBMB
Feeding LDL receptor (LDLR)-null mice a Western diet (WD) increased the expression of
IFN-β in jejunum as determined by quantitative RT-PCR (RT-qPCR), immunohistochemistry
(IHC), and ELISA (all P< 0.0001). WD also increased the expression of cholesterol 25-
hydroxylase (CH25H) as measured by RT-qPCR (P< 0.0001), IHC (P= 0.0019), and ELISA
(P< 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as
determined by LC-MS/MS (P< 0.0001). Adding ezetimibe at 10 mg/kg/day or adding a …
IFN-β in jejunum as determined by quantitative RT-PCR (RT-qPCR), immunohistochemistry
(IHC), and ELISA (all P< 0.0001). WD also increased the expression of cholesterol 25-
hydroxylase (CH25H) as measured by RT-qPCR (P< 0.0001), IHC (P= 0.0019), and ELISA
(P< 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as
determined by LC-MS/MS (P< 0.0001). Adding ezetimibe at 10 mg/kg/day or adding a …