Fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours
JA Raleigh, GG Miller, AJ Franko, CJ Koch… - British journal of …, 1987 - nature.com
JA Raleigh, GG Miller, AJ Franko, CJ Koch, AF Fuciarelli, DA Kelly
British journal of cancer, 1987•nature.comPolyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-
activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical
studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in
patterns similar to those revealed by autoradiographic studies with a tritium-labelled
derivative of the fluorinated misonidazole analogue.
activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical
studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in
patterns similar to those revealed by autoradiographic studies with a tritium-labelled
derivative of the fluorinated misonidazole analogue.
Abstract
Polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.
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