Nrf2, a basic leucine zipper transcription factor, is an essential activator of the coordinated
transcription of genes encoding antioxidant enzymes and phase II detoxifying enzymes
through the regulatory sequence termed antioxidant response element (ARE). Recently we
reported evidence for the involvement of protein kinase C (PKC) in phosphorylating Nrf2 and
triggering its nuclear translocation in response to oxidative stress. We show here that
phosphorylation of purified rat Nrf2 by the catalytic subunit of PKC was blocked by a …