Acetate as a carbon source for lipid synthesis in cultured cells
BV Howard - Biochimica et Biophysica Acta (BBA)-Lipids and Lipid …, 1977 - Elsevier
Lipid synthesis from acetate and glucose has been studied in fibroblast cultures grown in
lipid free medium containing various concentrations of acetate and glucose. Their role in
lipid synthesis was evaluated by comparing the specific activities of total cell lipid, and sterol
and fatty acid subfractions, to that of the carbon source in the medium. When glucose was
present at 1000 μg/ml (5.6 mM) and acetate at 100 μg/ml (1.2 mM), the majority of the total
cell lipid and subfractions were derived from glucose. As acetate concentrations increased …
lipid free medium containing various concentrations of acetate and glucose. Their role in
lipid synthesis was evaluated by comparing the specific activities of total cell lipid, and sterol
and fatty acid subfractions, to that of the carbon source in the medium. When glucose was
present at 1000 μg/ml (5.6 mM) and acetate at 100 μg/ml (1.2 mM), the majority of the total
cell lipid and subfractions were derived from glucose. As acetate concentrations increased …