Comparative transcriptome pairwise analysis of spontaneously transformed multipotent stromal cells from human adipose tissue

DO Omelchenko, AA Rzhaninova… - Russian journal of …, 2014 - Springer
DO Omelchenko, AA Rzhaninova, DV Goldshtein
Russian journal of genetics, 2014Springer
Potential markers and the mechanism of the spontaneous transformation of multipotent
stromal cells (MSC) with perivascular immunophenotype have been determined. A
transcriptome comparative study was performed involving six paired specimens of normal
and spontaneously transformed MSC with perivascular immunophenotype, obtained in the
first passages following the isolation of adipose tissue of six healthy donors. According to the
results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of …
Abstract
Potential markers and the mechanism of the spontaneous transformation of multipotent stromal cells (MSC) with perivascular immunophenotype have been determined. A transcriptome comparative study was performed involving six paired specimens of normal and spontaneously transformed MSC with perivascular immunophenotype, obtained in the first passages following the isolation of adipose tissue of six healthy donors. According to the results obtained using the microarray Illumina HT-12 v4 with the Significance Analysis of Microarrays software, differentially expressed transcripts were revealed and a statistical analysis using Gene Ontology and Molecular Signatures Databases was conducted. The association of the spontaneous transformation of isolated MSC with perivascular immunophenotype with previously identified oncogenic cell transformation pathways (E2F, ATR/ATM, RAS, and RHOA) is suggested and further aims for more detailed study are set. Potential transformation markers, including largely unknown genes and those previously not associated with cancer genes (HSPB6, PLAC9, FEZ1, DTWD1, APH1A, and ATP5L), are described.
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