Isolation and characterization of human muscle-derived cells

SH Lu, CF Wei, AH Yang, MB Chancellor, LS Wang… - Urology, 2009 - Elsevier
OBJECTIVES: To isolate and characterize human muscle-derived cells (MDCs) for future
management applications on lower urinary tract symptoms, including stress urinary
incontinence and bladder reconstitution. The development of muscle stem cells for
transplantation or gene transfer in patients with muscle disorders has become more
attractive and challenging recently. METHODS: Human MDCs were isolated from the
skeletal muscles of the limbs. The muscle tissues were minced, digested at 37° C by 0.2 …