Hepatitis B virus genome recycling and de novo secondary infection events maintain stable cccDNA levels
C Ko, A Chakraborty, WM Chou, J Hasreiter… - Journal of …, 2018 - Elsevier
Background & Aims Several steps in the HBV life cycle remain obscure because of a lack of
robust in vitro infection models. These steps include particle entry, formation and
maintenance of covalently closed circular (ccc) DNA, kinetics of gene expression and viral
transmission routes. This study aimed to investigate infection kinetics and cccDNA dynamics
during long-term culture. Methods We selected a highly permissive HepG2-NTCP-K7 cell
clone engineered to express sodium taurocholate co-transporting polypeptide (NTCP) that …
robust in vitro infection models. These steps include particle entry, formation and
maintenance of covalently closed circular (ccc) DNA, kinetics of gene expression and viral
transmission routes. This study aimed to investigate infection kinetics and cccDNA dynamics
during long-term culture. Methods We selected a highly permissive HepG2-NTCP-K7 cell
clone engineered to express sodium taurocholate co-transporting polypeptide (NTCP) that …