[HTML][HTML] Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism
K Hara, K Yonezawa, QP Weng, MT Kozlowski… - Journal of Biological …, 1998 - ASBMB
The present study identifies the operation of a signal tranduction pathway in mammalian
cells that provides a checkpoint control, linking amino acid sufficiency to the control of
peptide chain initiation. Withdrawal of amino acids from the nutrient medium of CHO-IR cells
results in a rapid deactivation of p70 S6 kinase and dephosphorylation of eIF-4E BP1, which
become unresponsive to all agonists. Readdition of the amino acid mixture quickly restores
the phosphorylation and responsiveness of p70 and eIF-4E BP1 to insulin. Increasing the …
cells that provides a checkpoint control, linking amino acid sufficiency to the control of
peptide chain initiation. Withdrawal of amino acids from the nutrient medium of CHO-IR cells
results in a rapid deactivation of p70 S6 kinase and dephosphorylation of eIF-4E BP1, which
become unresponsive to all agonists. Readdition of the amino acid mixture quickly restores
the phosphorylation and responsiveness of p70 and eIF-4E BP1 to insulin. Increasing the …