[HTML][HTML] PTPN2 controls differentiation of CD4+ T cells and limits intestinal inflammation and intestinal dysbiosis

MR Spalinger, S Kasper, C Chassard, T Raselli… - Mucosal …, 2015 - nature.com
MR Spalinger, S Kasper, C Chassard, T Raselli, I Frey-Wagner, C Gottier, S Lang, K Atrott…
Mucosal immunology, 2015nature.com
Loss-of-function variants within the gene locus encoding protein tyrosine phosphatase non-
receptor type 2 (PTPN2) are associated with increased risk for Crohn's disease (CD). A
disturbed regulation of T helper (Th) cell responses causing loss of tolerance against self-or
commensal-derived antigens and an altered intestinal microbiota plays a pivotal role in CD
pathogenesis. Loss of PTPN2 in the T-cell compartment causes enhanced induction of Th1
and Th17 cells, but impaired induction of regulatory T cells (Tregs) in several mouse colitis …
Abstract
Loss-of-function variants within the gene locus encoding protein tyrosine phosphatase non-receptor type 2 (PTPN2) are associated with increased risk for Crohn’s disease (CD). A disturbed regulation of T helper (Th) cell responses causing loss of tolerance against self-or commensal-derived antigens and an altered intestinal microbiota plays a pivotal role in CD pathogenesis. Loss of PTPN2 in the T-cell compartment causes enhanced induction of Th1 and Th17 cells, but impaired induction of regulatory T cells (Tregs) in several mouse colitis models, namely acute and chronic dextran sodium sulfate colitis, and T-cell transfer colitis models. This results in increased susceptibility to intestinal inflammation and intestinal dysbiosis which is comparable with that observed in CD patients. We detected inflammatory infiltrates in liver, kidney, and skin and elevated autoantibody levels indicating systemic loss of tolerance in PTPN2-deficient animals. CD patients featuring a loss-of-function PTPN2 variant exhibit enhanced Th1 and Th17 cell, but reduced Treg markers when compared with PTPN2 wild-type patients in serum and intestinal tissue samples. Our data demonstrate that dysfunction of PTPN2 results in aberrant T-cell differentiation and intestinal dysbiosis similar to those observed in human CD. Our findings indicate a novel and crucial role for PTPN2 in chronic intestinal inflammation.
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