The concentrations of dihydroxyacetone phosphate (DHAP), glycerol- 3-phosphate (GP), glucose and alkylglycerols were measured in bovine colostrum and milk. Concentrations of GP were higher in colostrum than in milk, and corresponded to intracellular concentrations at the onset of lactation. This suggests that GP equilibrates over the apical membrane at onset of lactation, which is believed to occur also in full lactation. The ratio of DHAP to GP showed a peak in colostrum that was more than twice that in milk, indicating that concentration changes in colostrum/milk were not caused by dilution. DHAP was positively correlated to metabolical compounds such as glucose and alkylglycerols. As DHAP is a required precursor for alkylglycerol synthesis, the positive correlation suggests that DHAP may be limiting for alkylglycerol synthesis and thus could explain the elevated levels of alkylglycerols found in colostrum.