[HTML][HTML] Neutrophil killing of Staphylococcus aureus in diabetes, obesity and metabolic syndrome: a prospective cellular surveillance study

IL Scully, LK McNeil, S Pathirana, CL Singer… - Diabetology & metabolic …, 2017 - Springer
IL Scully, LK McNeil, S Pathirana, CL Singer, Y Liu, S Mullen, D Girgenti, A Gurtman…
Diabetology & metabolic syndrome, 2017Springer
Abstract Background Obesity, metabolic syndrome (MetS), and diabetes are frequent in
surgical populations and can enhance susceptibility to postoperative surgical site infections.
Reduced neutrophil function has been linked with diabetes and risk of Staphylococcus
aureus infection. Therefore, neutrophil function in diabetic and obese subjects (±MetS) was
assessed in this prospective serological and cellular surveillance study to determine
whether vaccines administered to protect against infections after surgery could be effective …
Background
Obesity, metabolic syndrome (MetS), and diabetes are frequent in surgical populations and can enhance susceptibility to postoperative surgical site infections. Reduced neutrophil function has been linked with diabetes and risk of Staphylococcus aureus infection. Therefore, neutrophil function in diabetic and obese subjects (± MetS) was assessed in this prospective serological and cellular surveillance study to determine whether vaccines administered to protect against infections after surgery could be effective in these populations.
Methods
Neutrophil function (chemotaxis, phagocytosis, and opsonophagocytic killing of S. aureus) was assessed in subjects classified according to diabetes status, body mass index, and presence/absence of MetS. Neutrophils were characterized within functional subsets by flow cytometry. A serologic assay was used to measure baseline antibody presence to each antigen in SA4Ag: capsular polysaccharide (CP) type 5, CP8, recombinant mutant Clumping factor A (rmClfA), and recombinant Manganese transport protein C (rMntC).
Results
Neutrophil function was similar for comorbid and healthy cohorts, with no significant between-group differences in cell counts, migration, phagocytosis ability, neutrophil subset proportions, and S. aureus killing ability when neutrophils were isolated 3–6 months apart (Visit 1 [n = 90] and Visit 2 [n = 70]) and assessed. Median pre-existing antibody titers to CP5, CP8, and rmClfA were comparable for all cohorts (insufficient subjects with rMntC titers for determination).
Conclusions
MetS, diabetes, and obesity do not impact in vitro neutrophil function with regard to S. aureus killing, suggesting that if an effective S. aureus vaccine is developed it may be effective in individuals with these comorbidities.
Springer