[HTML][HTML] Subregion-and cell type–restricted gene knockout in mouse brain
Cell, 1996•cell.com
Using the phage P1–derived Cre/loxP recombination system, we have developed a method
to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to
a subregion or a specific cell type in the brain such as the pyramidal cells of the
hippocampal CA1 region. The Cre/loxP recombination–based gene deletion appears to
require a certain level of Cre protein expression. The brain subregional restricted gene
knockout should allow a more precise analysis of the impact of a gene mutation on animal …
to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to
a subregion or a specific cell type in the brain such as the pyramidal cells of the
hippocampal CA1 region. The Cre/loxP recombination–based gene deletion appears to
require a certain level of Cre protein expression. The brain subregional restricted gene
knockout should allow a more precise analysis of the impact of a gene mutation on animal …
Abstract
Using the phage P1–derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination–based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.
cell.com