[PDF][PDF] Neutrophils transport antigen from the dermis to the bone marrow, initiating a source of memory CD8+ T cells

D Duffy, H Perrin, V Abadie, N Benhabiles… - Immunity, 2012 - cell.com
D Duffy, H Perrin, V Abadie, N Benhabiles, A Boissonnas, C Liard, B Descours…
Immunity, 2012cell.com
The bone marrow (BM) has been identified as a possible organ for T cell priming, yet the
fundamental mechanisms of a polyclonal immune response in the BM remain unknown. We
found that after intradermal injection of modified vaccinia Ankara virus, unexpected sources
of newly primed polyclonal virus-specific CD8+, but not CD4+, T cells were localized in the
BM and the draining lymph nodes (dLNs) prior to blood circulation. We identified neutrophils
as the virus-carrier cells from the dermis to the BM. In both neutrophil-depleted and Ccr1 …
Summary
The bone marrow (BM) has been identified as a possible organ for T cell priming, yet the fundamental mechanisms of a polyclonal immune response in the BM remain unknown. We found that after intradermal injection of modified vaccinia Ankara virus, unexpected sources of newly primed polyclonal virus-specific CD8+, but not CD4+, T cells were localized in the BM and the draining lymph nodes (dLNs) prior to blood circulation. We identified neutrophils as the virus-carrier cells from the dermis to the BM. In both neutrophil-depleted and Ccr1−/− mice, virus-specific BM CD8+ responses were lost. Myeloid antigen-presenting cells were required for BM CD8+ T cell priming. A systems biology analysis of dLN and BM virus-specific CD8+ T cells revealed distinct transcriptional and multifunctional profiles for cells primed in each organ. We provide direct evidence for how antigen is transported to the BM, providing a source of virus-specific memory CD8+ T cells.
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