[PDF][PDF] Anti-interleukin-9 antibody increases the effect of allergen-specific immunotherapy in murine allergic rhinitis

JH Shin, DH Kim, BY Kim, SW Kim… - Allergy, asthma & …, 2017 - synapse.koreamed.org
JH Shin, DH Kim, BY Kim, SW Kim, SH Hwang, J Lee, SW Kim
Allergy, asthma & immunology research, 2017synapse.koreamed.org
Purpose Interleukin (IL)-9 induces allergic responses; however, the roles of anti-IL-9
antibody in the induction of tolerance remain unclear. This study investigated the effects of
anti-IL-9 antibody on oral tolerance (OT) in a mouse model of allergic rhinitis (AR). Methods
BALB/c mice were divided into 4 groups: the control, AR, OT, and OT with anti-IL-9 antibody
(OT+ IL9AB) groups. Ovalbumin (OVA) was used for sensitization and challenge. Mice in the
OT and OT+ IL9AB groups were fed OVA for immunotherapy. During immunotherapy, OT+ …
Purpose
Interleukin (IL)-9 induces allergic responses; however, the roles of anti-IL-9 antibody in the induction of tolerance remain unclear. This study investigated the effects of anti-IL-9 antibody on oral tolerance (OT) in a mouse model of allergic rhinitis (AR).
Methods
BALB/c mice were divided into 4 groups: the control, AR, OT, and OT with anti-IL-9 antibody (OT+IL9AB) groups. Ovalbumin (OVA) was used for sensitization and challenge. Mice in the OT and OT+IL9AB groups were fed OVA for immunotherapy. During immunotherapy, OT+IL9AB mice were injected with anti-IL-9 antibody. Allergic symptoms, tissue eosinophil counts, and serum OVA-specific immunoglobulin E (IgE) were measured. The mRNA expressions of cytokines and transcription factors of T cells of nasal mucosa were determined by real-time polymerase chain reaction (PCR). The protein levels of GATA3, ROR-γt, and Foxp3 in nasal mucosa were determined by Western blot. CD4+CD25+Foxp3+ T cells in the spleen were analyzed by flow cytometry.
Results
Administration of anti-IL-9 antibody decreased allergic symptoms, OVA-specific IgE levels, and eosinophil counts. In addition, it inhibited T-helper (Th) 2 responses, but had no effect on Th1 responses. Protein levels of ROR-γt and mRNA levels of PU.1 and ROR-γt were reduced by anti-IL-9 antibody. Anti-IL-9 antibody increased Foxp3 and IL-10 mRNA expression, Foxp3 protein, and induction of CD4+CD25+Foxp3+ T cells.
Conclusions
Anti-IL-9 antibody decreased allergic inflammation through suppression of Th2 and Th17 cells. Anti-IL-9 antibody enhanced the tolerogenic effects of regulatory T cells. These results suggest that anti-IL-9 antibody might represent a potential therapeutic agent for allergen immunotherapy in patients with uncontrolled allergic airway disease.
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