Asthma-associated differences in microbial composition of induced sputum
PR Marri, DA Stern, AL Wright, D Billheimer… - Journal of Allergy and …, 2013 - Elsevier
BACKGROUND: It is increasingly evident that microbial colonization of the respiratory tract
might have a role in the pathogenesis of asthma. OBJECTIVE: We sought to characterize
and compare the microbiome of induced sputum in asthmatic and nonasthmatic adults.
METHODS: Induced sputum samples were obtained from 10 nonasthmatic subjects and 10
patients with mild active asthma (8/10 were not using inhaled corticosteroids). Total DNA
was extracted from sputum supernatants and amplified by using primers specific for the V6 …
might have a role in the pathogenesis of asthma. OBJECTIVE: We sought to characterize
and compare the microbiome of induced sputum in asthmatic and nonasthmatic adults.
METHODS: Induced sputum samples were obtained from 10 nonasthmatic subjects and 10
patients with mild active asthma (8/10 were not using inhaled corticosteroids). Total DNA
was extracted from sputum supernatants and amplified by using primers specific for the V6 …