Human hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, an intricate,
multifactorial network of components producing cytokines, growth factors, and extracellular
matrix. The ability of HSCs to remain quiescent, self-renew or differentiate, and acquire
mutations and become malignant depends upon the complex interactions they establish
with different stromal components. To observe the crosstalk between human HSCs and the
human BM niche in physiological and pathological conditions, we designed a protocol to …