The micronucleus technique has been proposed as a method for measurement of
chromosomal damage in mitogen-stimulated human lymphocytes. Micronuclei require one
cell division to be expressed and, consequently, the conventional micronucleus technique is
very imprecise since the cells which have undergone only one division, and the micronuclei
in them, cannot be identified separately from the total population of lymphocytes. To
overcome this problem, two methods were developed to identify cells which have …