[HTML][HTML] Deletion of the C-terminal phosphorylation sites in the cardiac β-subunit does not affect the basic β-adrenergic response of the heart and the Cav1. 2 channel
J Brandmayr, M Poomvanicha, K Domes, J Ding… - Journal of Biological …, 2012 - ASBMB
Phosphorylation of the cardiac β subunit (Ca v β 2) of the Ca v 1.2 L-type Ca 2+ channel
complex has been proposed as a mechanism for regulation of L-type Ca 2+ channels by
various protein kinases including PKA, CaMKII, Akt/PKB, and PKG. To test this hypothesis
directly in vivo, we generated a knock-in mouse line with targeted mutation of the Ca v β 2
gene by insertion of a stop codon after proline 501 in exon 14 (mouse sequence Cacnb2;
βStop mouse). This mutation prevented translation of the Ca v β 2 C terminus that contains …
complex has been proposed as a mechanism for regulation of L-type Ca 2+ channels by
various protein kinases including PKA, CaMKII, Akt/PKB, and PKG. To test this hypothesis
directly in vivo, we generated a knock-in mouse line with targeted mutation of the Ca v β 2
gene by insertion of a stop codon after proline 501 in exon 14 (mouse sequence Cacnb2;
βStop mouse). This mutation prevented translation of the Ca v β 2 C terminus that contains …