[HTML][HTML] Checkpoints in adenoviral production: cross-contamination and E1A

DJ Haeussler, AM Evangelista, JR Burgoyne… - PLoS …, 2011 - journals.plos.org
DJ Haeussler, AM Evangelista, JR Burgoyne, RA Cohen, MM Bachschmid, DR Pimental
PLoS One, 2011journals.plos.org
Adenoviruses are widely used for overexpressing proteins in primary mammalian cells.
Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during
amplification, and identification of these products is crucial as the transcription of unwanted
genetic material can impact cell function and compromise data interpretation. Here we report
methods for evaluation of contaminating adenovirus and E1 viral DNA.
Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.
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